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recombinant human wnt-7a protein  (PeproTech)


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    PeproTech recombinant human wnt-7a protein
    Recombinant Human Wnt 7a Protein, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+wnt-7a+protein/recombinant+human+wnt7a/pmc11126970__pnas__2316006121__sapp-60-32-36
    Average 90 stars, based on 1 article reviews
    recombinant human wnt-7a protein - by Bioz Stars, 2026-09
    90/100 stars

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    Recombinant:

    Article Title: Supporting Information for Synergistic induction of blood-brain barrier properties
    Article Snippet: Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 μM) Ro 20-1724 (Sigma-Aldrich, cat# 557502, 17.5 μM), recombinant human Wnt-3a protein (R&D, cat# 5036-WN, 10-300 ng/mL), recombinant human Wnt-7a protein (PeproTech, cat# 120-31, 10-300 ng/mL), lithium chloride (LiCl; Sigma-Aldrich, cat# L9650, 3-30 mM), RepSox (PeproTech, cat# 4463325, 1-30 μM), A83-01 (PeproTech, cat# 9094360 or Tocris, cat# 2939, 0.1-3 μM) and the impedance of monolayers was measured at 10 kHz at every 5 min for 48-96 h. The readout of impedance measurements is cell index, a dimensionless parameter which correlates with the strength of cell-cell contacts and cell adhesion.

    Article Title: A small molecule cocktail for robust induction of blood-brain barrier properties
    Article Snippet: Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 μM) Ro 20-1724 (Sigma-Aldrich, cat# 557502, 17.5 μM), recombinant human Wnt-3a protein (R&D, cat# 5036-WN, 10-300 ng/mL), recombinant human Wnt-7a protein (PeproTech, cat# 120-31, 10-300 ng/mL), lithium chloride (LiCl; Sigma-Aldrich, cat# L9650, 3-30 mM), RepSox (PeproTech, cat# 4463325, 1-30 μM), A83-01 (PeproTech, cat# 9094360, 0.1-3 μM) and the impedance of monolayers was measured at 10 kHz at every 5 min for 48-96 h. For combinatorial treatments, the following concentrations were used: cAMP 250 μM, Ro 17.5 μM, Wnt-3a 300 ng/mL, Wnt-7a 300 ng/mL, LiCl 3 mM, RepSox 10 μM and A83-01 3 μM.

    Immunostaining:

    Article Title: Supporting Information for Synergistic induction of blood-brain barrier properties
    Article Snippet: Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 μM) Ro 20-1724 (Sigma-Aldrich, cat# 557502, 17.5 μM), recombinant human Wnt-3a protein (R&D, cat# 5036-WN, 10-300 ng/mL), recombinant human Wnt-7a protein (PeproTech, cat# 120-31, 10-300 ng/mL), lithium chloride (LiCl; Sigma-Aldrich, cat# L9650, 3-30 mM), RepSox (PeproTech, cat# 4463325, 1-30 μM), A83-01 (PeproTech, cat# 9094360 or Tocris, cat# 2939, 0.1-3 μM) and the impedance of monolayers was measured at 10 kHz at every 5 min for 48-96 h. The readout of impedance measurements is cell index, a dimensionless parameter which correlates with the strength of cell-cell contacts and cell adhesion.

    Article Title: A small molecule cocktail for robust induction of blood-brain barrier properties
    Article Snippet: Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 μM) Ro 20-1724 (Sigma-Aldrich, cat# 557502, 17.5 μM), recombinant human Wnt-3a protein (R&D, cat# 5036-WN, 10-300 ng/mL), recombinant human Wnt-7a protein (PeproTech, cat# 120-31, 10-300 ng/mL), lithium chloride (LiCl; Sigma-Aldrich, cat# L9650, 3-30 mM), RepSox (PeproTech, cat# 4463325, 1-30 μM), A83-01 (PeproTech, cat# 9094360, 0.1-3 μM) and the impedance of monolayers was measured at 10 kHz at every 5 min for 48-96 h. For combinatorial treatments, the following concentrations were used: cAMP 250 μM, Ro 17.5 μM, Wnt-3a 300 ng/mL, Wnt-7a 300 ng/mL, LiCl 3 mM, RepSox 10 μM and A83-01 3 μM.



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